Document Type
Article
Version
Final Published Version
Publication Title
Journal of Inorganic Biochemistry
Volume
249
Publication Date
2023
Abstract
Eight [Ru(bpy)2L]2+ and three [Ru(phen)2L]2+ complexes (where bpy = 2,2′-bipyridine and phen = 1,10-phenanthrolineare ancillary ligands, and L = a polypyridyl experimental ligand) were investigated for their G-quadruplex binding abilities. Fluorescence resonance energy transfer melting assays were used to screen these complexes for their ability to selectively stabilize human telomeric DNA variant, Tel22. The best G-quadruplex stabilizers were further characterized for their binding properties (binding constant and stoichiometry) using UV–vis, fluorescence spectroscopy, and mass spectrometry. The ligands’ ability to alter the structure of Tel22 was determined via circular dichroism and PAGE studies. We identified me2allox as the experimental ligand capable of conferring excellent stabilizing ability and good selectivity to polypyridyl Ru(II) complexes. Replacing bpy byphen did not significantly impact interactions with Tel22, suggesting that binding involves mostly the experimental ligand. However, using a particular ancillary ligand can help fine-tune G-quadruplex-binding propertie sof Ru(II) complexes. Finally, the fluorescence “light switch” behavior of all Ru(II) complexes in the presence ofTel22 G-quadruplex was explored. All Ru(II) complexes displayed “light switch” properties, especially [Ru(bpy)2(diamino)]2+, [Ru(bpy)2(dppz)]2+, and [Ru(bpy)2(aap)]2+. Current work sheds light on how Ru(II) polypyridyl complexes interact with human telomeric DNA with possible application in cancer therapy or Gquadruplex sensing.
Citation
Tran, Vienna T., Joshua Turek-Herman, Michelle Ferreira, Kailey N. Martin, Dana Beseiso, Benjamin R. Williams, Frederic Rosu, Valérie Gabelica, Sharon J. Nieter Burgmayer, and Liliya A. Yatsunyk. 2023. “Interactions of Ruthenium(II) Polypyridyl Complexes with Human Telomeric DNA.” Journal of Inorganic Biochemistry 249: 112388. https://doi.org/10.1016/j.jinorgbio.2023.112388.
DOI
https://doi.org/10.1016/j.jinorgbio.2023.112388
